tnf α inducible secreted alkaline phosphatase seap reporter gene  (InvivoGen)

Journal: Toxicology Reports

Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

doi: 10.1016/j.toxrep.2024.101883

Figure Lengend Snippet: Human cell lines used in this study (ATCC: American Type Culture Collection. EMEM: Eagle’s minimum essential medium. FBS: fetal bovine serum).

Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

Techniques: Activity Assay

Journal: Toxicology Reports

Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

doi: 10.1016/j.toxrep.2024.101883

Figure Lengend Snippet: Cytotoxic evaluation (EC 50 ) in µM following 24 h exposures of several indole metabolites on different cell lines: enterocytes (Caco-2), hepatocarcinoma cells (HepG2), hepatocytes (HepaRG), lung fibroblasts (MRC-5), mesenchymal stem cells (MSC), and breast epithelial (T47D). Data are presented as average ± standard deviation (n = 8–16). IPA: Indole-3-propionic acid. IAA: Indole-3-acetic acid. I3CA: Indole-3-carboxylic acid. I3A: Indole-3-carboxaldehyde. 3-MI: 3-Methylindole.

Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

Techniques: Standard Deviation

Journal: Toxicology Reports

Article Title: Exploring the biological impact of bacteria-derived indole compounds on human cell health: Cytotoxicity and cell proliferation across six cell lines

doi: 10.1016/j.toxrep.2024.101883

Figure Lengend Snippet: Aryl hydrocarbon receptor (AhR) activation by indole metabolites (IPA: indole-3-propionic acid; IAA: indole-3-acetic acid; I3CA: indole-3-carboxylic acid; I3A: indole-3-carbaldehyde; 3-MI: 3-methylindole; Indole: indole) using the HepG2-Lucia™ AhR gene-reporter cells. Data obtained from positive controls are also shown (TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; BaP: benzo[ a ]pyrene; BNF: β-naphthoflavone). Data are presented as EC 50 and average (95 % Confidence Interval) (n = 12–18).

Article Snippet: HepG2-LUCIA™ , HepG2 reporter gene cells for AhR activity , InvivoGen , EMEM + 10 % FBS + 1X non-essential amino acids medium + 100 μg/mL Normocin + 100 μg/mL Zeocin , Gao et al., .

Techniques: Activation Assay

Journal: Cell Reports Medicine

Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation

doi: 10.1016/j.xcrm.2022.100818

Figure Lengend Snippet:

Article Snippet: QUANTI-Luc Lucia luciferase reporter gene system , invivogen , rep-qlc2.

Techniques: Clinical Proteomics, Recombinant, Magnetic Beads, Cell Stimulation, Staining, Electron Microscopy, Purification, Sequencing, Modification, Luciferase, Liquid Chromatography, Mass Spectrometry, Software, Sterility, Suspension